Sample Set Annotation Tool: Modified natural and stimulated in vitro ferlitization cycle: Cumulus cells-GSE43684

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Cumulus cells surrounding mature oocytes that developed to moruale/blastocyst stage on day 5 of IVF cycle were collected and used for gene expression profiling using Affymetrix Human Gene 1.0 ST Arrays in order to determine differences in gene expression between the modified natural and stimulated in vitro fertilization (IVF) procedures.

Hypothesis

Experimental Design

Microarray analysis was made on 8 individual CC samples, derived from 5 patients that first underwent MNIVF, followed by a controlled ovarian hyperstimulation (COH) cycle. As the quality of the isolated RNA derived from 2 of the MNIVF CC samples was not good, these samples were not included in the analysis. Altogether, 3 CC samples from MNIVF and 5 CC samples from COH cycles were included in microarray analysis.

Experimental Variables

Controls

Cumulus cells from natural IVF

Methods

Additional Information

Expected Samples:

8 Samples Loaded: 8

Species

Human (Homo sapiens)

Sample Source

cumulus cells

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	group	patient	stimulus	Cell id
GSM1068180	COH, patient AH	Stimulated IVF	AH	GnRH Agonist	3
GSM1068181	COH, patient AU	Stimulated IVF	AU	GnRH Agonist	7
GSM1068182	COH, patient D	Stimulated IVF	D	GnRH Antagonist	1
GSM1068183	COH, patient J	Stimulated IVF	J	GnRH Antagonist	2
GSM1068184	COH, patient L	Stimulated IVF	L	GnRH Agonist	10

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File
sampleset4000027_sampleannotations-modified.csv Sample Set Spreadsheet
sampleset4000027_sampleannotations-modified.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Group	Patient	Stimulus	Cell id	Ivf procedure	Oocyte maturity
GSM1068180	COH, patient AH	Stimulated IVF	AH	GnRH Agonist	3	COH (controlled ovarian hyperstimulation) IVF	MII-blastocyst
GSM1068181	COH, patient AU	Stimulated IVF	AU	GnRH Agonist	7	COH (controlled ovarian hyperstimulation) IVF	MII-blastocyst
GSM1068182	COH, patient D	Stimulated IVF	D	GnRH Antagonist	1	COH (controlled ovarian hyperstimulation) IVF	MII-blastocyst
GSM1068183	COH, patient J	Stimulated IVF	J	GnRH Antagonist	2	COH (controlled ovarian hyperstimulation) IVF	MII-blastocyst
GSM1068184	COH, patient L	Stimulated IVF	L	GnRH Agonist	10	COH (controlled ovarian hyperstimulation) IVF	MII-blastocyst
GSM1068185	MNIVF, patient AU	Modified-Natural IVF	AU	none	s	MNIVF (modified natural IVF)	MII-blastocyst
GSM1068186	MNIVF, patient AH	Modified-Natural IVF	AH	none	s	MNIVF (modified natural IVF)	MII-morulae
GSM1068187	MNIVF, patient D	Modified-Natural IVF	D	none	s	MNIVF (modified natural IVF)	MII-morulae

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